Lipidomics Standards Initiative (LSI)
LSI aims to create guidelines for the major lipidomic workflows in a community-wide effort.
The guidelines/standards include the various
analytical steps in a lipidomics workflow, guiding how to (i) collect and store
samples, (ii) extract lipids, (iii) execute the MS analysis, (iv) perform data
processing, including lipid identification, deconvolution, annotation,
quantification, and evaluation of quality control, and (v) how to report the
data. The guidelines also cover the validation of analytical methods and the
use of quality controls.
Standards are essential for a successful progress of lipidomics because they provide a common language for researchers within lipdomics and the interface to interlink to other disciplines. Therefore LSI closely interacts with the other interest groups within the International Lipidomics Society. Moreover, LSI established collaborations with LIPID MAPS (https://www.lipidmaps.org/) and is in exchange with other standardization initiatives such as proteomics (PSI) and metabolomics (MSI) standards initiatives.
The LSI starts a series of online discussion meetings to promote guideline development: Preanalytics and Lipid Extraction, 15th of July 16:00 CET Direct Infusion MS,
Lipidomics needs more standardization Modern mass spectrometric technologies provide quantitative readouts for a wide variety of lipid specimens. However, many studies do not report absolute